KMID : 1094720190240040632
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Biotechnology and Bioprocess Engineering 2019 Volume.24 No. 4 p.632 ~ p.637
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Enhanced Production of Protopanaxatriol from Ginsenoside Re and Rg1 Using a Recombinant Bacterial ¥â-glucosidase
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Siddiqi Muhammad Zubair
Jin Meifang Song Bong-Kyu Park Hye-Yoon Oh Jung-Mi Chun Sung-Kun Jin Fengxie Yu Hongshan Im Wan-Taek
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Abstract
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20(S)-protopanaxatriol (PPTA), an aglycone of ginseng saponins, is rarely found and is present in a limited amount; it exhibits various pharmaceutical activities, including memory improvement, and anti-diabetic and anti-cancer effects. An enzymatic method was developed to improve 20(S)-protopanaxatriol aglycone [20(S)-PPTA] production using recombinant ¥â-glucosidase (BgpA) cloned from Terrabacter ginsenosidimutans Gsoil 3082T. The optimal hydrolytic activity was obtained at pH 7.0 and 37¡ÆC. The enzyme could hydrolyze the rutinoside and glucose moieties at the C6 and C20 positions, respectively, of ginsenosides Re and Rg1 to produce 20(S)-PPTA through the formation of ginsenoside F1 as an intermediate. For gram-scale production, the enzyme reaction was performed in a 10-L fermenter for 48 h at a substrate concentration of 10 mg/mL. Finally, 5.45 g 20(S)-PPTA was produced from 36 g PPT type ginsenoside mixture with 95% purity by chromatography. This study thus reveals an industrial application of a minor component, 20(S)-PPTA.
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KEYWORD
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ginsenoside, protopanaxatriol aglycone, ¥â-glucosidase, Terrabacter ginsenosidimutans, biotransformation
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